A chemical extention of both the hydroxyethyl amide and ZIC chemistries iHILIC FUSION FUSION offer features of both of the above approaches. Separation selectivity is favored by the 11 zwitterion charge balance which makes the ZIC-HILIC column overall neutral with weak but important ionic interactions.
Chromatograms Of Bleomycin Obtained Using Zic Hilic Stationary Phase Download Scientific Diagram
Separation selectivity is favored by the 11 zwitterion charge balance which makes the ZIC -HILIC Column overall neutral with weak but important ionic interactions.
Zic hilic stationary phase. 20 vv 100 mM NH 4 Ac buffer pH 45. Mm Column Length mm Guard Column 50 100 150 250 PEEK 35µm Phase 539 704 747 - 441 21 1504450001 1504470001 1504480001 - 15043400011 5pk 589 - 747 - 559 46 1504460001 - 1504490001 - 15043600012 3pk 5µm Phase 490 642 678 823 441. The silica-based ZIC-HILIC sorbent has a bonded stationary phase consisting of a highly polar permanent zwitterion.
Some of the zwitterionic phases are commercialized under the trademarks ZIC-HILIC ZIC-cHILIC KS-polyMPC Obelisc R and Obelisc N. Different classes of stationary phases for HILIC. Separation of methacryl-amide and acrylamide on a ZIC-HILIC column 250 x 46 mm 5 um 200 Å PN.
ZIC is a registered trademark of Merck KGaA The functional group of the ZIC-HILIC and ZICpHILIC stationary phases. At their turn these interactions depend on the composition of the mobile phase and temperature 59. Syncronis HILIC stationary phase carries a zwitterionic functional group of the sulfobetaine type monomerically bonded to highly pure high surface area silica.
Matysová L1 Havlíková L Hájková R Krivda A Solich P. Schematic illustation of the inverted zwitterionic ZIC-cHILIC cholinate stationary phase. Of polar analytes on HILIC stationary phase depends on the intesity of polar interactions between polar groups of analytes and polar moieties from stationary phase 34.
Zwitterionc liquid chromatography ZIC-HILIC is a particular HILIC. Ted ZIC -HILIC stationary phase. Peptide retention time prediction models have been developed for zwitter-ionic ZIC-HILIC and ZIC-cHILIC stationary phases pH 45 eluents using proteomics-derived retention datasets of.
MS-ESI- with SIM at 1172 mz Drying-gas flow rate Drying-gas temperature Capillary voltage. The retention processes in HILIC illustrated by hydrophilic partitioning and. Zwitterionic HILIC stationary phases are particularly versatile and they can be considered the HILIC all purpose phases.
The HILIC stationary phases SPs like Normal Phase Liquid Chromatography NPLC contain bare silica or modified silica gels with different polar functional groups Some designed various SPs such as diol TSK gel Amide-80 amino ZIC HILIC Click maltose and other polar ligands especially for HILIC. ZIC-HILIC Analytical PEEK Columns 35µm and 5µm 200Å Column id. Neutral top charged middle and zwitterionic bottom.
Application of HILIC stationary phase to determination of dimethindene maleate in topical gel. 10 Lmin 300C 30 kV. The selectivity of the ZIC-HILIC column can be tuned by varying the amount of organic modifier the pH and the low ionic strength of the eluent where an increase in the organic solvent content or a decrease in the ionic strength promotes retention.
Refractive index cell 9 μL 40C. The zwitterionic ZIC-HILIC column provided better chromatographic performance over the silica stationary phase with 14 standards achieving good quality peaks compared to the 7 with the silica column. ZIC-HILIC 100 x 21 mm 35 μm100 Å 1504440001 Mobile Phase.
The silica-based ZIC -HILIC sorbent has a bonded stationary phase consisting of a highly polar permanent zwitterion. 1Department of Analytical Chemistry Charles University Hradec Králové Czech Republic. The sulfobetaine zwitterion has both positive quaternary ammonium and negative sulfonic acid groups in a.
Only 2 standards were undetected with the ZIC-HILIC column compared to the 14 undetected with the silica column. In principle HILIC stationary phases can be divided into three different groups neutral charged and zwitterionic. The amide column is based on the ethylene bridged hybrid BEH particle technology with trifunctionally-bonded amide phase.
Schematic illustation of the zwitterionic ZIC-HILIC betain sulfonate stationary phase. The ZIC-HILIC column contains a zwitterionic sulfoalkylbetaine stationary phase covalently attached to porous silica.
Hilic Mz Analysentechnik Gmbh
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